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nsc proliferation medium  (Beijing Solarbio Science)


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    Beijing Solarbio Science nsc proliferation medium
    Preparation and bioactivity evaluation of immobilized recombinant growth factors and the construction of the neuroregenerative coating fiber network scaffold. (A) SDS-PAGE gel showed the preparation process of immobilized recombinant growth factors D-IGF1 and D-NGF including: marker (M), pre-induction (1), post-induction (2), supernatant after centrifugation (3), pellet after centrifugation (4), refolded protein solution (5), flow-through liquid (6), wash liquid (7), elution liquid (8). (B) The effect of different densities of D-IGF1 and D-NGF on <t>NSC</t> <t>proliferation.</t> (C–D) The effect of different densities of D-IGF1 and D-NGF on neural stem cell differentiation. Scale bars, 100 μm (E) SEM images of the surface morphology of fiber scaffolds prepared under different process parameters. Scale bars, 10 μm (F–G) Biocompatibility testing of the scaffold. (H) Contact angle analysis. (I) FT-IR analysis. (J) XPS analysis. (Data are shown as the mean ± SD, n = 3, ∗p < 0.05,∗∗p < 0.01).
    Nsc Proliferation Medium, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 99/100, based on 11142 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nsc+proliferation+medium/Medium/pmc12357159-340-6-34
    Average 99 stars, based on 11142 article reviews
    nsc proliferation medium - by Bioz Stars, 2026-11
    99/100 stars

    Images

    1) Product Images from "Neuroactive network tissue based on dual-factor neuroregenerative bioactive coating scaffolds and neural stem cells for spinal cord injury repair"

    Article Title: Neuroactive network tissue based on dual-factor neuroregenerative bioactive coating scaffolds and neural stem cells for spinal cord injury repair

    Journal: Materials Today Bio

    doi: 10.1016/j.mtbio.2025.102172

    Preparation and bioactivity evaluation of immobilized recombinant growth factors and the construction of the neuroregenerative coating fiber network scaffold. (A) SDS-PAGE gel showed the preparation process of immobilized recombinant growth factors D-IGF1 and D-NGF including: marker (M), pre-induction (1), post-induction (2), supernatant after centrifugation (3), pellet after centrifugation (4), refolded protein solution (5), flow-through liquid (6), wash liquid (7), elution liquid (8). (B) The effect of different densities of D-IGF1 and D-NGF on NSC proliferation. (C–D) The effect of different densities of D-IGF1 and D-NGF on neural stem cell differentiation. Scale bars, 100 μm (E) SEM images of the surface morphology of fiber scaffolds prepared under different process parameters. Scale bars, 10 μm (F–G) Biocompatibility testing of the scaffold. (H) Contact angle analysis. (I) FT-IR analysis. (J) XPS analysis. (Data are shown as the mean ± SD, n = 3, ∗p < 0.05,∗∗p < 0.01).
    Figure Legend Snippet: Preparation and bioactivity evaluation of immobilized recombinant growth factors and the construction of the neuroregenerative coating fiber network scaffold. (A) SDS-PAGE gel showed the preparation process of immobilized recombinant growth factors D-IGF1 and D-NGF including: marker (M), pre-induction (1), post-induction (2), supernatant after centrifugation (3), pellet after centrifugation (4), refolded protein solution (5), flow-through liquid (6), wash liquid (7), elution liquid (8). (B) The effect of different densities of D-IGF1 and D-NGF on NSC proliferation. (C–D) The effect of different densities of D-IGF1 and D-NGF on neural stem cell differentiation. Scale bars, 100 μm (E) SEM images of the surface morphology of fiber scaffolds prepared under different process parameters. Scale bars, 10 μm (F–G) Biocompatibility testing of the scaffold. (H) Contact angle analysis. (I) FT-IR analysis. (J) XPS analysis. (Data are shown as the mean ± SD, n = 3, ∗p < 0.05,∗∗p < 0.01).

    Techniques Used: Recombinant, SDS Page, Marker, Centrifugation, Cell Differentiation

    Related Articles

    Recombinant:

    Article Title: Neuroactive network tissue based on dual-factor neuroregenerative bioactive coating scaffolds and neural stem cells for spinal cord injury repair
    Article Snippet: The tissue suspension was filtered sequentially through 70 μm and 40 μm cell strainers, and the filtrate was centrifuged at 1500 rpm for 5 min at room temperature.The tissue suspension was filtered sequentially through 70 μm and 40 μm cell strainers, and the filtrate was centrifuged at 1500 rpm for 5 min at room temperature.. The resulting cells were resuspended in NSC proliferation medium containing DMEM/F12 (Gibco, A4192001, USA), 1 % penicillin-streptomycin (PS, Sigma-Aldrich, USA), 1 % glutamine (Gibco, USA), 20 ng mL −1 basic fibroblast growth factor (bFGF, Solarbio, China), and 20 ng mL −1 epidermal growth factor (EGF, Solarbio, China).. Cells were cultured at 37 °C in a 5 % CO 2 incubator.Cells were cultured at 37 °C in a 5 % CO 2 incubator.

    SDS Page:

    Article Title: Neuroactive network tissue based on dual-factor neuroregenerative bioactive coating scaffolds and neural stem cells for spinal cord injury repair
    Article Snippet: The tissue suspension was filtered sequentially through 70 μm and 40 μm cell strainers, and the filtrate was centrifuged at 1500 rpm for 5 min at room temperature.The tissue suspension was filtered sequentially through 70 μm and 40 μm cell strainers, and the filtrate was centrifuged at 1500 rpm for 5 min at room temperature.. The resulting cells were resuspended in NSC proliferation medium containing DMEM/F12 (Gibco, A4192001, USA), 1 % penicillin-streptomycin (PS, Sigma-Aldrich, USA), 1 % glutamine (Gibco, USA), 20 ng mL −1 basic fibroblast growth factor (bFGF, Solarbio, China), and 20 ng mL −1 epidermal growth factor (EGF, Solarbio, China).. Cells were cultured at 37 °C in a 5 % CO 2 incubator.Cells were cultured at 37 °C in a 5 % CO 2 incubator.

    Marker:

    Article Title: Neuroactive network tissue based on dual-factor neuroregenerative bioactive coating scaffolds and neural stem cells for spinal cord injury repair
    Article Snippet: The tissue suspension was filtered sequentially through 70 μm and 40 μm cell strainers, and the filtrate was centrifuged at 1500 rpm for 5 min at room temperature.The tissue suspension was filtered sequentially through 70 μm and 40 μm cell strainers, and the filtrate was centrifuged at 1500 rpm for 5 min at room temperature.. The resulting cells were resuspended in NSC proliferation medium containing DMEM/F12 (Gibco, A4192001, USA), 1 % penicillin-streptomycin (PS, Sigma-Aldrich, USA), 1 % glutamine (Gibco, USA), 20 ng mL −1 basic fibroblast growth factor (bFGF, Solarbio, China), and 20 ng mL −1 epidermal growth factor (EGF, Solarbio, China).. Cells were cultured at 37 °C in a 5 % CO 2 incubator.Cells were cultured at 37 °C in a 5 % CO 2 incubator.

    Centrifugation:

    Article Title: Neuroactive network tissue based on dual-factor neuroregenerative bioactive coating scaffolds and neural stem cells for spinal cord injury repair
    Article Snippet: The tissue suspension was filtered sequentially through 70 μm and 40 μm cell strainers, and the filtrate was centrifuged at 1500 rpm for 5 min at room temperature.The tissue suspension was filtered sequentially through 70 μm and 40 μm cell strainers, and the filtrate was centrifuged at 1500 rpm for 5 min at room temperature.. The resulting cells were resuspended in NSC proliferation medium containing DMEM/F12 (Gibco, A4192001, USA), 1 % penicillin-streptomycin (PS, Sigma-Aldrich, USA), 1 % glutamine (Gibco, USA), 20 ng mL −1 basic fibroblast growth factor (bFGF, Solarbio, China), and 20 ng mL −1 epidermal growth factor (EGF, Solarbio, China).. Cells were cultured at 37 °C in a 5 % CO 2 incubator.Cells were cultured at 37 °C in a 5 % CO 2 incubator.

    Cell Differentiation:

    Article Title: Neuroactive network tissue based on dual-factor neuroregenerative bioactive coating scaffolds and neural stem cells for spinal cord injury repair
    Article Snippet: The tissue suspension was filtered sequentially through 70 μm and 40 μm cell strainers, and the filtrate was centrifuged at 1500 rpm for 5 min at room temperature.The tissue suspension was filtered sequentially through 70 μm and 40 μm cell strainers, and the filtrate was centrifuged at 1500 rpm for 5 min at room temperature.. The resulting cells were resuspended in NSC proliferation medium containing DMEM/F12 (Gibco, A4192001, USA), 1 % penicillin-streptomycin (PS, Sigma-Aldrich, USA), 1 % glutamine (Gibco, USA), 20 ng mL −1 basic fibroblast growth factor (bFGF, Solarbio, China), and 20 ng mL −1 epidermal growth factor (EGF, Solarbio, China).. Cells were cultured at 37 °C in a 5 % CO 2 incubator.Cells were cultured at 37 °C in a 5 % CO 2 incubator.



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    Image Search Results


    Preparation and bioactivity evaluation of immobilized recombinant growth factors and the construction of the neuroregenerative coating fiber network scaffold. (A) SDS-PAGE gel showed the preparation process of immobilized recombinant growth factors D-IGF1 and D-NGF including: marker (M), pre-induction (1), post-induction (2), supernatant after centrifugation (3), pellet after centrifugation (4), refolded protein solution (5), flow-through liquid (6), wash liquid (7), elution liquid (8). (B) The effect of different densities of D-IGF1 and D-NGF on NSC proliferation. (C–D) The effect of different densities of D-IGF1 and D-NGF on neural stem cell differentiation. Scale bars, 100 μm (E) SEM images of the surface morphology of fiber scaffolds prepared under different process parameters. Scale bars, 10 μm (F–G) Biocompatibility testing of the scaffold. (H) Contact angle analysis. (I) FT-IR analysis. (J) XPS analysis. (Data are shown as the mean ± SD, n = 3, ∗p < 0.05,∗∗p < 0.01).

    Journal: Materials Today Bio

    Article Title: Neuroactive network tissue based on dual-factor neuroregenerative bioactive coating scaffolds and neural stem cells for spinal cord injury repair

    doi: 10.1016/j.mtbio.2025.102172

    Figure Lengend Snippet: Preparation and bioactivity evaluation of immobilized recombinant growth factors and the construction of the neuroregenerative coating fiber network scaffold. (A) SDS-PAGE gel showed the preparation process of immobilized recombinant growth factors D-IGF1 and D-NGF including: marker (M), pre-induction (1), post-induction (2), supernatant after centrifugation (3), pellet after centrifugation (4), refolded protein solution (5), flow-through liquid (6), wash liquid (7), elution liquid (8). (B) The effect of different densities of D-IGF1 and D-NGF on NSC proliferation. (C–D) The effect of different densities of D-IGF1 and D-NGF on neural stem cell differentiation. Scale bars, 100 μm (E) SEM images of the surface morphology of fiber scaffolds prepared under different process parameters. Scale bars, 10 μm (F–G) Biocompatibility testing of the scaffold. (H) Contact angle analysis. (I) FT-IR analysis. (J) XPS analysis. (Data are shown as the mean ± SD, n = 3, ∗p < 0.05,∗∗p < 0.01).

    Article Snippet: The resulting cells were resuspended in NSC proliferation medium containing DMEM/F12 (Gibco, A4192001, USA), 1 % penicillin-streptomycin (PS, Sigma-Aldrich, USA), 1 % glutamine (Gibco, USA), 20 ng mL −1 basic fibroblast growth factor (bFGF, Solarbio, China), and 20 ng mL −1 epidermal growth factor (EGF, Solarbio, China).

    Techniques: Recombinant, SDS Page, Marker, Centrifugation, Cell Differentiation

    Journal: iScience

    Article Title: An exercise “sweet spot” reverses cognitive deficits of aging by growth-hormone-induced neurogenesis

    doi: 10.1016/j.isci.2021.103275

    Figure Lengend Snippet:

    Article Snippet: Following centrifugation at 100 × g for 5 min, the cells were re-suspended in 1 ml of Neurocult NSC Basal Medium-plus Proliferation Supplement (Stem Cell Technologies), supplemented with 20 ng/ml epidermal growth factor (EGF; BD Biosciences), 10 ng/ml basic fibroblast growth factor (bFGF; Roche) and 20 ng/ml heparin (Sigma-Aldrich), referred to as the control (E + F) condition.

    Techniques: Recombinant, Fluorescence, Enzyme-linked Immunosorbent Assay, Knock-In, Software